¹Ì»ý¹° µ¿Á¤ ¹æ¹ýÀÇ ºñ±³¿Í Æò°¡: ¹ÚÅ׸®¾Æ µ¿Á¤ÀÇ ÃÖÀûÈ­ µÈ ½Ã½ºÅÛ MALDI-Biotyper!
 
 
MALDI Biotyper
  1. 59ÁÖÀÇ CF¿¡¼­ 57ÁÖÀÇ ±ÕÁ¾ ¹× ±Õ¼ÓÀÌ È®ÀÎÀÌ µÇ¾úÀ¸¸ç ±ÕÁ¾Àº 50.8 %, ±Õ¼ÓÀº 45.8 %°¡ µ¿Á¤ µÇ¾ú´Ù.
  2. 46ÁÖÀÇ non-CF¿¡¼­ 44ÁÖ°¡ µ¿Á¤ µÇ¾úÀ¸¸ç 63.0 %°¡ ±ÕÁ¾ ¼öÁØÀ¸·Î µ¿Á¤ µÇ¾úÀ¸¸ç, 26.1 %°¡ ±Õ¼Ó ¼öÁØÀ¸·Î µ¿Á¤ÀÌ µÇ¾ú´Ù.
Phoenix
  1. 1. Phoenix¸¦ ÀÌ¿ëÇÑ µ¿Á¤°á°ú·Î´Â CF¿¡¼­ 25.4 %°¡ ±ÕÁ¾ ¼öÁرîÁö µ¿Á¤ÀÌ µÇ¾úÀ¸¸ç 64.4%°¡ ±Õ¼Ó ¼öÁرîÁö µ¿Á¤ÀÌ µÇ¾ú´Ù. (Table 1).
  2. 2. Non-CF¿¡¼­´Â, 58.7 %°¡ ±ÕÁ¾ ¼öÁØ 30.4 % ±Õ¼Ó ¼öÁرîÁö µ¿Á¤ÀÌ µÇ¾ú´Ù. (Table 2).


Table 1. Identification results of CF isolates for three methods

1.  Cryseobacterium ureilyticum was not included in both MALDI Biotyper and Phoenix databases.
2.  Pandorea was not included in Phoenix database
3.  Ralstonia insidiosa was not included in Phoenix database and 15/17 isolates were identified as Ralstonia pickettii.


Table 2. Identification results of non-CF isolates for three methods

1.  Brevibacillus borstelensis, Bordetella hinzii and Paenibacillus spp were not included in Phoenix database.
2.  Brevindomonas terrae was not included in MALDI-TOFF MS database.
3.  Pseudomonas plecoglossicida was not included in both MALDI Biotyper and Phoenix databases.
Table 3Àº MALDI Biotyper¿Í Phoenix SystemÀ» ÀÌ¿ëÇÑ µ¿Á¤·üÀ» ºñ±³ÇÑ Ç¥ÀÌ´Ù.
Table 3Àº MALDI Biotyper¿Í Phoenix SystemÀ» ÀÌ¿ëÇÑ µ¿Á¤·üÀ» ºñ±³ÇÑ Ç¥ÀÌ´Ù.
Reference ºÐ¼®¹ý(16s rRNA gene sequencing)°ú ºñ±³ÇÏ¿© MALDI ºÐ¼®¹ýÀº 93.3 %ÀÇ ÀÏÄ¡À²À» º¸¿´°í Phoenix ºÐ¼®¹ýÀº 89.5 %ÀÇ ÀÏÄ¡À²À» º¸¿´´Ù.


Table 3: Identification rates of MALDI Biotyper and
Phoenix System compared to sequencing for CF and non-CF isolates

Chryseobacterium ureilyticum (n:2)
Ralstonia insidiosa (n:2), Chryseobacterium ureilyticum (n:2), Chryseobacterium spp. (n:1), Pandoreae pulmonicola (n:1)
**  Paenibacillus spp. (n:2), Pseudomonas spp. (n:1), Brevundimonas terrae (n:1), Pseudomonas plecoglossicida (n:1)
##  Paenibacillus spp. (n:2), Bordetella hinzii (n:1), Pseudomonas spp. (n:1), Brevibacillus borstelensis (n:1)

MALDI Biotyper´Â °ª½Î°í È¿À²ÀûÀÎ ¹æ¹ýÀ̸ç, ¸¹Àº Á¾ ´Ù¾ç¼ºÀÌ Á¸ÀçÇÏ´Â CF¿¡¼­µµ Á¤È®ÇÏ°í ºü¸¥ µ¿Á¤ÀÌ °¡´ÉÇÏ´Ù´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù.
Conventional method¿Í ºñ±³ÇÏ¿© MALDI Biotyper¸¦ ÀÌ¿ëÇÑ µ¿Á¤ ¹ýÀº ºñ±³ °ËÁõ¿¡¼­ ´õ ³ôÀº ½Å·Úµµ¸¦ º¸¿´´Ù.
Á¤È®ÇÏ°í ¸¹Àº µ¿Á¤À» À§ÇÏ¿© MALDI BiotyperÀÇ score °ªÀÇ thresholds¸¦ ÃÖÀûÈ­ÇÒ ¼÷Á¦°¡ ³²¾ÆÀÖ´Ù. »õ·Î¿î cutoff values¿Í ÇÔ²² ³·Àº threshold °ªÀ» ÀÌ¿ë ÇÏ¸é ´õ¿í ¸¹°í Á¤È®ÇÑ µ¿Á¤À» ÇÒ ¼ö ÀÖÀ» °ÍÀÌ¶ó ¿¹»óµÈ´Ù.