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MALDI Biotyper |
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1. |
59ÁÖÀÇ CF¿¡¼ 57ÁÖÀÇ ±ÕÁ¾ ¹× ±Õ¼ÓÀÌ È®ÀÎÀÌ µÇ¾úÀ¸¸ç ±ÕÁ¾Àº 50.8 %, ±Õ¼ÓÀº 45.8 %°¡ µ¿Á¤ µÇ¾ú´Ù. |
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2. |
46ÁÖÀÇ non-CF¿¡¼ 44ÁÖ°¡ µ¿Á¤ µÇ¾úÀ¸¸ç 63.0 %°¡ ±ÕÁ¾ ¼öÁØÀ¸·Î µ¿Á¤ µÇ¾úÀ¸¸ç, 26.1 %°¡ ±Õ¼Ó ¼öÁØÀ¸·Î µ¿Á¤ÀÌ µÇ¾ú´Ù. |
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Phoenix |
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1. |
1. Phoenix¸¦ ÀÌ¿ëÇÑ µ¿Á¤°á°ú·Î´Â CF¿¡¼ 25.4 %°¡ ±ÕÁ¾ ¼öÁرîÁö µ¿Á¤ÀÌ µÇ¾úÀ¸¸ç 64.4%°¡ ±Õ¼Ó ¼öÁرîÁö µ¿Á¤ÀÌ µÇ¾ú´Ù. (Table 1). |
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2. Non-CF¿¡¼´Â, 58.7 %°¡ ±ÕÁ¾ ¼öÁØ 30.4 % ±Õ¼Ó ¼öÁرîÁö µ¿Á¤ÀÌ µÇ¾ú´Ù. (Table 2). |
Table 1. Identification results of CF isolates for three methods
1. |
Cryseobacterium ureilyticum was not included in both MALDI Biotyper and Phoenix databases. |
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2. |
Pandorea was not included in Phoenix database |
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3. |
Ralstonia insidiosa was not included in Phoenix database and 15/17 isolates were identified as Ralstonia pickettii. |
Table 2. Identification results of non-CF isolates for three methods
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Brevibacillus borstelensis, Bordetella hinzii and Paenibacillus spp were not included in Phoenix database. |
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2. |
Brevindomonas terrae was not included in MALDI-TOFF MS database. |
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3. |
Pseudomonas plecoglossicida was not included in both MALDI Biotyper and Phoenix databases. |
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Table 3Àº MALDI Biotyper¿Í Phoenix SystemÀ» ÀÌ¿ëÇÑ µ¿Á¤·üÀ» ºñ±³ÇÑ Ç¥ÀÌ´Ù. |
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Table 3Àº MALDI Biotyper¿Í Phoenix SystemÀ» ÀÌ¿ëÇÑ µ¿Á¤·üÀ» ºñ±³ÇÑ Ç¥ÀÌ´Ù. |
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Reference ºÐ¼®¹ý(16s rRNA gene sequencing)°ú ºñ±³ÇÏ¿© MALDI ºÐ¼®¹ýÀº 93.3 %ÀÇ ÀÏÄ¡À²À» º¸¿´°í Phoenix ºÐ¼®¹ýÀº 89.5 %ÀÇ ÀÏÄ¡À²À» º¸¿´´Ù. |
Table 3: Identification rates of MALDI Biotyper and
Phoenix System compared to sequencing for CF and non-CF isolates
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Chryseobacterium ureilyticum (n:2) |
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Ralstonia insidiosa (n:2), Chryseobacterium ureilyticum (n:2), Chryseobacterium spp. (n:1), Pandoreae pulmonicola (n:1) |
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Paenibacillus spp. (n:2), Pseudomonas spp. (n:1), Brevundimonas terrae (n:1), Pseudomonas plecoglossicida (n:1) |
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Paenibacillus spp. (n:2), Bordetella hinzii (n:1), Pseudomonas spp. (n:1), Brevibacillus borstelensis (n:1) |
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MALDI Biotyper´Â °ª½Î°í È¿À²ÀûÀÎ ¹æ¹ýÀ̸ç, ¸¹Àº Á¾ ´Ù¾ç¼ºÀÌ Á¸ÀçÇÏ´Â CF¿¡¼µµ Á¤È®ÇÏ°í ºü¸¥ µ¿Á¤ÀÌ °¡´ÉÇÏ´Ù´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù. |
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Conventional method¿Í ºñ±³ÇÏ¿© MALDI Biotyper¸¦ ÀÌ¿ëÇÑ µ¿Á¤ ¹ýÀº ºñ±³ °ËÁõ¿¡¼ ´õ ³ôÀº ½Å·Úµµ¸¦ º¸¿´´Ù. |
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Á¤È®ÇÏ°í ¸¹Àº µ¿Á¤À» À§ÇÏ¿© MALDI BiotyperÀÇ score °ªÀÇ thresholds¸¦ ÃÖÀûÈÇÒ ¼÷Á¦°¡ ³²¾ÆÀÖ´Ù. »õ·Î¿î cutoff values¿Í ÇÔ²² ³·Àº threshold °ªÀ» ÀÌ¿ë ÇÏ¸é ´õ¿í ¸¹°í Á¤È®ÇÑ µ¿Á¤À» ÇÒ ¼ö ÀÖÀ» °ÍÀÌ¶ó ¿¹»óµÈ´Ù. |
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