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ÅÍÅ°ÀÇ Ankara¿¡ ÀÖ´Â ÇÑ ´ëÇк´¿ø ¹Ì»ý¹° °Ë»ç½Ç¿¡¼ ¹è¾çµÈ ±ÕÁÖ·Î ½ÇÇèÀ» ¼öÇàÇÏ¿´À¸¸ç ÃÑ 105ÁÖÀÇ NFGNB(CF ȯÀÚ 59ÁÖ, non-CF ȯÀÚ 46ÁÖ)À» ´ë»óÀ¸·Î ÇÏ¿´´Ù. |
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Automated Identification System (Becton Dickinson Diagnostics, MD, USA)°ú MALDI Biotyper (Bruker Daltonics, Bremen, Germany)¸¦ ÀÌ¿ëÇÏ¿© µ¿Á¤ ºñ±³½ÇÇèÀ» ¼öÇàÇÏ¿´´Ù. (figure2). |
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16S rDNA gene sequencing (ABI PRISM 3100, Applied Biosystems, USA) À» ÀÌ¿ëÇÏ¿© °á°úÀÇ ºñ±³ È®ÀÎ ÀÛ¾÷À» ¼öÇàÇÏ¿´´Ù. |
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Biotyper system¿¡¼ score cut-offs °ªÀº ¸ÞÀÌÄ¿¿¡¼ ÁöÁ¤ÇÑ ¼Ó ¼öÁØ 2 ÀÌ»ó, Á¾ ¼öÁØ 1.7 ~ 2 ¹üÀ§·Î ÁöÁ¤ÇÏ¿´´Ù. |
Figure 2.Workflow for bacteria identification using MALDI Biotyper.
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