[½ÅÁ¦Ç°] PerkinElmer»ç TGA 8000 ¸ðµ¨ Ãâ½Ã!!
 
 
Results and discussion
Ink analysis
   DESI¸¦ ÀÌ¿ëÇÏ¿© ¿°·á¿¡ Á¸ÀçÇÏ´Â À×Å© ¼ººÐÀÇ È­ÇÐÀûÀÎ ±¸Á¶¸¦ ¹àÇô³¾ ¼ö ÀÖ¾úÀ¸¸ç, ÀçÇö¼º Ãø¸é¿¡¼­µµ ù ¹ø° ½ºÄµ°ú 100¹ø° ½ºÄµ ÀüÈÄ sample ¼Ò¸ð Â÷ÀÌ´Â °ÅÀÇ ¾ø¾ú´Ù. Results and discussion
   Fig1. DESI·Î ºÐ¼®ÇÑ ink ÀÇ ÁÖ¿ä ¼ººÐ ±¸Á¶Fig1. DESI·Î ºÐ¼®ÇÑ ink ÀÇ ÁÖ¿ä ¼ººÐ ±¸Á¶Fig2. Ææ F ·Î ±×¸° 4°³ ÆòÇ༱ÀÇ 1 ¹ø° ½ºÄµ(A1) °ú 100 ¹ø° ½ºÄµ(A2) ½Ã°¢Àû À̹ÌÁö; Basic Blue 7, m/z478ÀÇ extracted ion chronogram ÀÇ 1 ¹ø° ½ºÄµ(B1), 100 ¹ø° ½ºÄµ(B2); total ion chronogram(0.28-0.32min) ÀÇ average mass spectra 1 ¹ø° ½ºÄµ(C1), 100 ¹ø° ½ºÄµ(C2); 30ºÐ µ¿¾È ÇÑ °÷¿¡¼­ ÂïÀº Basic Blue 7 ÀÇ spray intensity (D).Fig2. Ææ F ·Î ±×¸° 4°³ ÆòÇ༱ÀÇ 1 ¹ø° ½ºÄµ(A1) °ú 100 ¹ø° ½ºÄµ(A2) ½Ã°¢Àû À̹ÌÁö; Basic Blue 7, m/z478ÀÇ extracted ion chronogram ÀÇ 1 ¹ø° ½ºÄµ(B1), 100 ¹ø° ½ºÄµ(B2); total ion chronogram(0.28-0.32min) ÀÇ average mass spectra 1 ¹ø° ½ºÄµ(C1), 100 ¹ø° ½ºÄµ(C2); 30ºÐ µ¿¾È ÇÑ °÷¿¡¼­ ÂïÀº Basic Blue 7 ÀÇ spray intensity (D).
Imaging
1.  Ææ C·Î 1432¸¦ ¾´ µÚ Ææ E·Î 43 ÀÚ¸®¿¡ 98À̶ó´Â ¼ýÀÚ·Î º¸ÀÌ°Ô µ¤¾î ¾²°í ³­ µÚ BioMap ¼ÒÇÁÆ®¿þ¾î¸¦ »ç¿ëÇÏ¿© µÎ À̹ÌÁö¸¦ merge ½ÃÅ°¸é 1982¶ó´Â ¼ýÀÚ·Î º¸ÀÌ°Ô µÈ´Ù. À̹Ì¡À¸·Î ºÃÀ» ¶§´Â 1982·Î º¸ÀÌÁö¸¸ ÀÌ°ÍÀ» DESI·Î ºÐ¼®ÇÏ°Ô µÇ¸é ¸í¹éÇÏ°Ô µÎ ÆæÀ» ±¸º°ÇÒ ¼ö ÀÖ´Ù.
   Fig3. DESI¸¦ ÀÌ¿ëÇÑ À§Á¶µÈ ¼ýÀÚ À̹ÌÁö. (A) Basic Violet 3, m/z 372 ÀÇ 2D- ion image (B) Solvent Blue 2, m/z 484 ÀÇ 2D-ion image (C) A¿Í BÀÇ overlap (D) Á¾ÀÌ À§ ½Ã°¢ÀûÀÎ À̹ÌÁö.Fig3. DESI¸¦ ÀÌ¿ëÇÑ À§Á¶µÈ ¼ýÀÚ À̹ÌÁö. (A) Basic Violet 3, m/z 372 ÀÇ 2D- ion image (B) Solvent Blue 2, m/z 484 ÀÇ 2D-ion image (C) A¿Í BÀÇ overlap (D) Á¾ÀÌ À§ ½Ã°¢ÀûÀÎ À̹ÌÁö.
2.  25³â µÈ ³ëÆ®ÀÇ Çʱ⸦ DESI¸¦ ÀÌ¿ëÇÏ¿© 2D-À̹Ì¡À¸·Î ³ªÅ¸³»¾ú°í °ËÀº»ö ¹è°æºÎºÐ°ú Èò»ö ±Û¾¾ ºÎºÐÀ» ÀÌ¿ÂÈ­ ÇÏ¿© ¾²¿©Áø ÆæÀÇ ¼ººÐÀ» ºÐ¼®ÇÒ ¼ö ÀÖ¾ú´Ù.
   Fig4. (A)1981³âµµ ¹®¼­ÀÇ ½Ã°¢Àû À̹ÌÁö (B) Basic Blue 7(m/z 478) ÀÇ 2D DESI image (C) °ËÀº ºÎºÐÀÇ full scan mass spectrum (D) ±Û¾¾ ºÎºÐÀÇ full scan massFig4. (A)1981³âµµ ¹®¼­ÀÇ ½Ã°¢Àû À̹ÌÁö (B) Basic Blue 7(m/z 478) ÀÇ 2D DESI image (C) °ËÀº ºÎºÐÀÇ full scan mass spectrum (D) ±Û¾¾ ºÎºÐÀÇ full scan mass
3.  ±¤ÇÐÀ̹ÌÁö·Î º¸ÀÌ´Â 1982¶ó´Â ±ÛÀÚ¸¦ solvent blue¸¸ extraction ÇÏ°í, basic violet 3 ¸¸À» extractionÇÏ¿© 9¿Í 8¿¡ ´ëÇÑ ±¸º°À» Çس¾ ¼ö ÀÖ¾ú´Ù.
   Fig5. À§Á¶µÈ ¼ýÀÚÀÇ screening (A) ½Ã°¢Àû À̹ÌÁö (B) Solvent Blue 2, m/z 484 ÀÇ Extracted ion chronogram (C) Basic Violet 3, m/z 372 ÀÇ Extracted ion chronogram.Fig5. À§Á¶µÈ ¼ýÀÚÀÇ screening (A) ½Ã°¢Àû À̹ÌÁö (B) Solvent Blue 2, m/z 484 ÀÇ Extracted ion chronogram (C) Basic Violet 3, m/z 372 ÀÇ Extracted ion chronogram.

Conclusion

°á·ÐÀûÀ¸·Î, DESI¸¦ ÀÌ¿ëÇÑ ¹ýÀÇÇÐÀû À̹Ì¡ ÀÀ¿ë ºÐ¾ß¿¡ ´ëÇÑ ¼º°øÀûÀÎ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ¾ú´Ù. ¿°·áºÐ¼®¿¡ À־ ¿©·¯ °¡Áö ¹æ¹ýµéÀº °¢°¢ÀÇ ÀåÁ¡°ú ´ÜÁ¡À» °¡Áö°í ÀÖ°í ÀÌ·¯ÇÑ ¹æ¹ýÀ» DESI¿Í °áÇÕÇÑ´Ù¸é ¿¹¸¦ µé¾î, DESI¿Í Çü±¤ºÐ¼®À» °áÇÕÇÏ¸é »óÈ£º¸¿ÏÀûÀÎ ½Ç½Ã°£ À̹Ì¡À» ±¸ÇöÇØ ³¾ ¼ö ÀÖ¾î °­·ÂÇÑ ¹ýÀÇÇÐÀû tool·Î ¾²ÀÏ °ÍÀÌ´Ù. DESI À̹Ì¡ÀÇ ÀåÁ¡Àº »ùÇÃÀÇ Àüó¸®, »ùÇÃÀ» ¿ÂÀüÈ÷ ÂïÀ» ¼ö ÀÖ´Ù´Â °Í¿¡ ÀåÁ¡ÀÌ ÀÖÀ¸¸ç À§Á¶µÈ ¹®¼­¿Í °°ÀÌ º¸Á¸ÇØ¾ß ÇÏ´Â »ùÇÃÀ» ºÐ¼®ÇØ¾ß ÇÏ´Â ¹ýÀÇÇÐ ºÐ¼®¿¡ ÀÖ¾î ¸Å¿ì À¯¿ëÇÑ ±â¼ú·Î ¾²ÀÏ ¼ö ÀÖ´Ù.